It is very important to understand the input features and the neural network parameters required for optimal path loss prediction in wireless communication channels. In this paper, an extensive investigation was conducted to determine the most appropriate neural network parameters for path loss prediction in Very High Frequency (VHF) band. Field measurements were conducted in an urban propagation environment to obtain relevant geographical and network information about the receiving mobile equipment and quantify the path losses of radio signals transmitted at 189.25 MHz and 479.25 MHz. Different neural network architectures were trained with varying kinds of input parameters, number of hidden neurons, activation functions, and learning algorithms to accurately predict corresponding path loss values. At the end of the experiments, the performance of the developed Artificial Neural Network (ANN) models are evaluated using the following statistical metrics: Mean Absolute Error (MAE), Mean Squared Error (MSE), Root Mean Squared Error (RMSE), Standard Deviation (SD) and Regression coefficient (R). Results obtained show that the ANN model that yielded the best performance employed four input variables (latitude, longitude, elevation, and distance), nine hidden neurons, hyperbolic tangent sigmoid (tansig) activation function, and the Levenberg-Marquardt (LM) learning algorithm with MAE, MSE, RMSE, SD and R values of 0.58 dB, 0.66 dB, 0.81 dB, 0.56 dB and 0.99 respectively. Finally, a comparative analysis of the developed model with Hata, COST 231, ECC-33 and Egli models showed that ANN-based path loss model has better prediction accuracy and generalization ability than the empirical models.